Name：HEPES
Cas: 7365-45-9
Purity: 99%
MOQ: 1KG
| Melting point | 234-238 °C |
| Boiling point | 408℃[at 101 325 Pa] |
| bulk density | 560kg/m3 |
| density | 1.07 g/mL at 20 °C |
| vapor pressure | 0Pa at 25℃ |
| refractive index | n20/D 1.339 |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25℃) |
| PH Range | 7.0 – 7.6 |
| biological source | synthetic |
| Water Solubility | Soluble |
Directory Guidance on HEPES
⇒We provide high-purity chemical raw materials with clear molecular formula and precise specifications. Each batch of HEPES products undergoes strict quality inspection to ensure stable and reliable physical and chemical properties. It adopts international standard packaging, sealed and moisture-proof, to ensure safe transportation and storage. Whether it is R&D experiments or industrial production, we can provide you with professional technical support and customized services. With rigorous technology, transparent data and stable supply, we help you efficiently complete chemical applications and innovations.
HEPES has actually evolved from an easy chemical reagent to among one of the most fundamental and crucial devices in modern-day life sciences, chemistry, and clinical research because of its exceptional pH buffering capacity, chemical inertness, low steel complexation properties, and excellent biocompatibility. It plays a supportive duty in several core areas such as cell culture, healthy protein study, and molecular biology, giving basic warranties for the reliability and success rate of numerous scientific research and biotechnology applications by keeping the acid-base homeostasis of the system.
HEPES is an indispensable buffering element in the field of cell culture, particularly in open society or speculative circumstances that need lasting monitoring. The conventional bicarbonate barrier system counts on a particular concentration of co2 atmosphere (normally 5% CARBON MONOXIDE TWO), and when it leaves the incubator, the pH of the society medium will sharply raise due to the quick retreat of carbon dioxide, causing alkaline stress to the cells. HEPES, as a potent chemical barrier, can individually maintain the pH of the culture medium within the physiological array (usually 7.2-7.4) without counting on the carbon monoxide two setting, thus playing a vital duty in supplying a secure acid-base microenvironment for cells, successfully ensuring their vigor and normal physiological functions during fluid exchange, control, or microscopic observation.
HEPES is extensively made use of in protein chemistry and biochemistry research as an experimental system for healthy protein filtration, enzyme kinetics evaluation, and healthy protein interactions. Due to its lack of significant complexation responses with a lot of steel ions, it is especially ideal for reaction systems that need divalent cations (such as Mg ² ⁺, Ca Two ⁺) to maintain enzyme activity, such as HEPES buffer used in the onset of PCR responses. On top of that, HEPES is inert, to ensure that it will not interfere with the framework and feature of the healthy protein itself, and can give a steady pH atmosphere for the protein, so regarding make sure the accuracy and repeatability of the experimental outcomes. It is an usual buffer option in crucial technologies such as gel electrophoresis, chromatographic separation and healthy protein formation.
HEPES is the basic buffering part of various essential reagents in molecular biology experiments. For instance, in cell transfection experiments, the commonly utilized HEPES buffer salt service (HBS) is the core reagent for preparing calcium phosphate DNA co speeds up, and its accurate pH control is essential for forming the needed fine precipitate bits for effective transfection. Furthermore, it is likewise contributed to particular nucleic acid hybridization barriers, electrophoresis barriers, and bacterial society media to supply a neutral and stable atmosphere for the task of DNA, RNA, and nucleases, ensuring effective and certain biochemical processes such as limitation enzyme food digestion and ligation reactions.
Related References
Manufacturer of HEPES
Zhishang Chemical is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional HEPES manufacturer, Zhi shang Chemical has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Pre-Sales Service
* Timely reply and 24 hours online, the professional team will provide you with the most favorable prices and high-quality products.
* The sample supports testing and inspection.
* Each batch of products will be tested to ensure that its quality meets user needs.
*Packaging can also be made according to customer requirements.
*Any inquiries will be answered by our relevant personnel within 24 hours.
*We will provide you with commercial invoice, packing list, packing list, COA, health certificate and certificate of origin if you need it. If your market has other special requirements, please let us know.
After-Sales Service
*We will monitor the logistics information in real time and will share the information with you.
* You can consult us at any time if you have any questions about the product, and we will answer you in time.
*If you have any questions about the product, you can report it to us, we will deal with it in time for you, and the product can be returned.
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
LEARN MORE ABOUT US
Shandong Look Chemical
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
Company Info
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